MAPK variant effect atlas

Proteins Interactions

Proteins / CRAF / interface with HSP90AB1

CRAF – HSP90AB1

PDB PDB 7Z38 (chains C/A) supported · 2 of 2 complexes CRAF volcano ↗

Colour CRAF (chain A) by

Interface statistics (basal)

Merged point of Fig. 6c,d: median Cohen's d of the complexes below; q is the BH-FDR of their geometric-mean p. A complex is supported at q < 0.05 and |d| ≥ 0.2. 6 interface and 95 control positions in the structure shown.

Structure shown: source

Experimental structure PDB 7Z38 · electron microscopy, 3.16 Å · chains C/A
Structure of the RAF1-HSP90-CDC37 complex (RHC-I)
Primary citation: Garcia-Alonso et al., Mol Cell 82 (2022)
RCSB Protein Data Bank (Berman et al., Nucleic Acids Res. 2000); the two chains were extracted and renumbered to UniProt (SIFTS) for this analysis.

Complexes merged into this interface

PDB 7Z38 (chains C/A) (shown)
PDB 8GAE (chains D/B)

Residue

Click a residue in the viewer.

Created by Sriram Pendyala with help from Claude Opus (Anthropic), based on the paper by Jessica J. Simon*, Sriram Pendyala*, …, Dustin J. Maly, Douglas M. Fowler (*equal contribution; preprint coming soon).

Code: GitHub (FowlerLab/MAPK-LABEL-seq) · Data: Zenodo (doi:10.5281/zenodo.23121367). Structures: RCSB PDB, Predictomes (AlphaFold-Multimer), Zhang et al. 2025 (RoseTTAFold2-PPI).