MAPK variant effect atlas

Proteins Interactions

Proteins / BRAF / interface with KRAS

BRAF – KRAS

PDB PDB 8DGS (chains E/A) supported · 2 of 2 complexes BRAF volcano ↗

Colour BRAF (chain B) by

Interface statistics (basal)

Merged point of Fig. 6c,d: median Cohen's d of the complexes below; q is the BH-FDR of their geometric-mean p. A complex is supported at q < 0.05 and |d| ≥ 0.2. 6 interface and 180 control positions in the structure shown.

Structure shown: source

Experimental structure PDB 8DGS · electron microscopy, 4.30 Å · chains E/A
Cryo-EM structure of a RAS/RAF complex (state 1)
Primary citation: Park et al., Nat Commun 14 (2023)
RCSB Protein Data Bank (Berman et al., Nucleic Acids Res. 2000); the two chains were extracted and renumbered to UniProt (SIFTS) for this analysis.

Complexes merged into this interface

PDB 8DGS (chains E/A) (shown)
PDB 8DGT (chains F/A)

Residue

Click a residue in the viewer.

Created by Sriram Pendyala with help from Claude Opus (Anthropic), based on the paper by Jessica J. Simon*, Sriram Pendyala*, …, Dustin J. Maly, Douglas M. Fowler (*equal contribution; preprint coming soon).

Code: GitHub (FowlerLab/MAPK-LABEL-seq) · Data: Zenodo (doi:10.5281/zenodo.23121367). Structures: RCSB PDB, Predictomes (AlphaFold-Multimer), Zhang et al. 2025 (RoseTTAFold2-PPI).