MAPK variant effect atlas

Proteins Interactions

Proteins / KRAS / interface with KRAS

KRAS – KRAS

PDB PDB 7O70 (chains A/D) · H_N_K_Ras_like #2 supported · 2 of 2 complexes KRAS volcano ↗

Colour KRAS (chain A) by

Interface statistics (basal)

Merged point of Fig. 6c,d: median Cohen's d of the complexes below; q is the BH-FDR of their geometric-mean p. A complex is supported at q < 0.05 and |d| ≥ 0.2. 4 interface and 70 control positions in the structure shown.

Structure shown: source

Experimental structure PDB 7O70 · X-ray, 1.18 Å · chains A/D
KRasG12C ligand complex
Primary citation: Kettle et al., J Med Chem 65 (2022)
RCSB Protein Data Bank (Berman et al., Nucleic Acids Res. 2000); the two chains were extracted and renumbered to UniProt (SIFTS) for this analysis.

Complexes merged into this interface

PDB 7O70 (chains A/D) · chain A (shown)
PDB 7O70 (chains A/D) · chain B

Residue

Click a residue in the viewer.

Created by Sriram Pendyala with help from Claude Opus (Anthropic), based on the paper by Jessica J. Simon*, Sriram Pendyala*, …, Dustin J. Maly, Douglas M. Fowler (*equal contribution; preprint coming soon).

Code: GitHub (FowlerLab/MAPK-LABEL-seq) · Data: Zenodo (doi:10.5281/zenodo.23121367). Structures: RCSB PDB, Predictomes (AlphaFold-Multimer), Zhang et al. 2025 (RoseTTAFold2-PPI).