MAPK variant effect atlas

Proteins Interactions

Proteins / EGFR / interface with ERBB3

EGFR – ERBB3

PDB PDB 4RIW (chains B/A) supported · 3 of 3 complexes EGFR volcano ↗

Colour EGFR (chain A) by

Interface statistics (basal)

Merged point of Fig. 6c,d: median Cohen's d of the complexes below; q is the BH-FDR of their geometric-mean p. A complex is supported at q < 0.05 and |d| ≥ 0.2. 22 interface and 90 control positions in the structure shown.

Structure shown: source

Experimental structure PDB 4RIW · X-ray, 3.10 Å · chains B/A
Crystal structure of an EGFR/HER3 kinase domain heterodimer
Primary citation: Littlefield et al., Sci Signal 7 (2014)
RCSB Protein Data Bank (Berman et al., Nucleic Acids Res. 2000); the two chains were extracted and renumbered to UniProt (SIFTS) for this analysis.

Complexes merged into this interface

PDB 4RIW (chains B/A) (shown)
PDB 4RIX (chains B/A)
PDB 4RIY (chains B/A)

Residue

Click a residue in the viewer.

Created by Sriram Pendyala with help from Claude Opus (Anthropic), based on the paper by Jessica J. Simon*, Sriram Pendyala*, …, Dustin J. Maly, Douglas M. Fowler (*equal contribution; preprint coming soon).

Code: GitHub (FowlerLab/MAPK-LABEL-seq) · Data: Zenodo (doi:10.5281/zenodo.23121367). Structures: RCSB PDB, Predictomes (AlphaFold-Multimer), Zhang et al. 2025 (RoseTTAFold2-PPI).