MAPK variant effect atlas

Proteins Interactions

Proteins / EGFR / interface with EGFR

EGFR – EGFR

PDB PDB 5CNO (chains B/A) · PTKc_EGFR #2 supported · 2 of 2 complexes EGFR volcano ↗

Colour EGFR (chain A) by

Interface statistics (basal)

Merged point of Fig. 6c,d: median Cohen's d of the complexes below; q is the BH-FDR of their geometric-mean p. A complex is supported at q < 0.05 and |d| ≥ 0.2. 4 interface and 118 control positions in the structure shown.

Structure shown: source

Experimental structure PDB 5CNO · X-ray, 1.55 Å · chains B/A
Crystal structure of the EGFR kinase domain mutant V924R
Primary citation: Kovacs et al., Mol Cell Biol 35 (2015)
RCSB Protein Data Bank (Berman et al., Nucleic Acids Res. 2000); the two chains were extracted and renumbered to UniProt (SIFTS) for this analysis.

Complexes merged into this interface

PDB 5CNO (chains B/A) · chain A (shown)
PDB 8PO4 (chains A/B) · chain A

Residue

Click a residue in the viewer.

Created by Sriram Pendyala with help from Claude Opus (Anthropic), based on the paper by Jessica J. Simon*, Sriram Pendyala*, …, Dustin J. Maly, Douglas M. Fowler (*equal contribution; preprint coming soon).

Code: GitHub (FowlerLab/MAPK-LABEL-seq) · Data: Zenodo (doi:10.5281/zenodo.23121367). Structures: RCSB PDB, Predictomes (AlphaFold-Multimer), Zhang et al. 2025 (RoseTTAFold2-PPI).